CANCER TEST
CARCINOMA OF UNKNOWN PRIMARY (CUP)
C.A.RE.
Information
Questions
Turn Around Time:
C.A.RE: 2 weeks*
* In case additional enrichment of the material is required, for the successful results of the tests, we will contact you.
I Choose C.A.RE
Rearrangements are a class of genomic alterations that are attractive both as therapeutic targets and as diagnostic tools, due to their intrinsic expression exclusively in cancer tissues. Already, in several cancers, rearrangements of specific genes are pharmacologically targetable, while several of them are found in more than one type of cancer, therefore they are characterized as tumor agnostic.
C.A.RE. was developed to offer fusion screening in 49 driver genes , while it can, in selected genes (ALK, FGFR2, NTRK1, NTRK2, NTRK3 and RET), identify novel rearrangements (i.e. beyond the known rearrangements specified in the literature).
Also, in selected genes (AR, BRAF, BRCA1, EGFR, MET, NTRK1 and RELA), CARE. can identify rearrangements outside the exons (intragenic exon rearrangements) and accurately predict some exon skipping.
Consult your Oncologist for the best choice and personalization for you. There is the possibility of further personalization of the tests.
In case your sample is not already at Microdiagnostics archive, please contact us immediately so that we can arrange for its safe and rapid transport to our laboratory. You will also need to quickly and easily complete the Consent Form.
C.A.RE. is recommended for all histological types of cancer, to find rearrangements (fusions) in genes that can be targeted pharmacologically.
The tests are performed on the surgical specimen (paraffin cubes) or the biopsy material (paraffin cube) from which your histological examination was performed or on the aspiration material (FNAB, EBUS) from which your cytological examination was performed. In our fully integrated Laboratory, the pathologist selects the most appropriate & representative paraffin cube, ensuring that the most appropriate sample will be used for the tests. Qualitative and quantitative parameters are checked.
In case your sample is not already at Microdiagnostics archive, please contact us immediately so that we can arrange for its safe and rapid transport to our laboratory. You will also need to quickly and easily complete the Consent Form.
In our fully integrated Laboratory, we handle your sample in such a way as to minimize its unnecessary waste:
- The paraffin cube is placed, if possible, once on the microtome for obtaining tissue sections, by experienced tissue technologists.
- Tissue sections are obtained sequentially in such a way as to ensure diagnosis and perform Immunohistochemistry if required.
- Ensuring preservation of tissue sections for further molecular examinations.
- Pathologists perform microdissection to ensure the maximum amount of cancer cells possible, removing all other necrotic cells or normal tissue that could affect the validity of the results.
- The molecular biologist immediately processes the tissue in a fully controlled environment, using next-generation sequencing (NGS) or real-time polymerase chain reaction (Real-time PCR) to identify mutations in the genes of interest.
Frequently Asked Questions (FAQ)
C.A.RE. can help any cancer patient detect any rearrangements (fusions) in key genes related to targeted therapies, offering a significant window of survival to the patient.
The tests are performed on the surgical specimen (paraffin cubes) or the biopsy material (paraffin cube) from which your histological examination was performed or on the aspiration material (FNAB, EBUS) from which your cytological examination was performed. In our fully integrated Laboratory, the pathologist selects the most appropriate & representative paraffin cube, ensuring that the most appropriate sample will be used for the tests. Qualitative and quantitative parameters are checked.
In case your sample is not already at Microdiagnostics archive, please contact us immediately so that we can arrange for its safe and rapid transport to our laboratory. You will also need to quickly and easily complete the Consent Form.
Most of the time, the sample material we are called upon to handle is small because it has resulted from a minimally invasive method (needle biopsy, fluid aspiration, paraffin block with minimal material).
In our laboratory, Pathologists check in a timely manner whether the material to be examined is sufficient. If so, then a management algorithm is followed, with the aim of achieving the performance of multiple tests on the material (Immunohistochemistry, real-time PCR, NGS) in order to fully check the molecular profile of your tumor (proteins, genes, histological Grading).
In this case, and once sample enrichment manipulations have been exhausted, we contact your clinician to discuss alternative approaches in order to obtain the desired information to select the optimal treatment for you. Some examples:
- Performing an alternative test (e.g. Immunohistochemistry instead of PCR, or choosing Next Generation Sequencing (NGS))
- Performing Immunohistochemistry instead of FISH (Fluorescent In Situ Hybridization) and tubulin
- Possible blood sampling instead of tissue testing (liquid biopsy)
- Possible option to take a new biopsy or puncture
Contact us at 2310 23 22 72 and we will immediately assist you in quickly transporting the sample to our laboratory.
By cash, bank card, bank deposit, or Online interbank deposit.
One of the primary concerns at Microdiagnostics is the protection of your personal data as well as the strict observance of the conditions for the protection of your genetic material and medical results.
In full compliance with the General Data Protection Regulation (GDPR), we ensure that any examination conducted is done with your knowledge and consent and we do not communicate results over the phone.